>With IBM tossing it’s hat into the ring of “next-next-generation” sequencing, I’m starting to get lost as to which generation is which. For the moment, I’m sort of lumping things together, while I wait to see how the field plays out. In my mind, first generation is anything that requires chain termination, Second generation is chemical based pyrosequencing, and third generation is single molecule sequencing based on a nano-scale mechanical process. It’s a crude divide, but it seems to have some consistency.
At any rate, I decided I’d collect a few videos to illustrate each one. For Sanger, there are a LOT of videos, many of which are quite excellent, but I only wanted one. (Sorry if I didn’t pick yours.) For second and third generation DNA sequencing videos, the selection kind of flattens out, and two of them come from corporate sites, rather than youtube – which seems to be the general consensus repository of technology videos.
Personally, I find it interesting to see how each group is selling themselves. You’ll notice some videos press heavily on the technology, while others focus on the workflow.
As an aside, I also find it interesting to look for places where the illustrations don’t make sense… there’s a lovely place in the 454 video where two strands of DNA split from each other on the bead, leaving the two full strands and a complete primer sequence… mysterious! (Yes, I do enjoy looking for inconsistencies when I go to the movies.)
Ok, get out your popcorn.
Sanger Entry: Link
Pyrosequencing Entry: Link
Helicose Entry: Link
Illumina (Corporate site): Link
454 Entry: Link
Pacific Biosciences: Link
Oxford Nanopore Entry: Link
IBM’s Entry: Link
Note: If I’ve missed something, please let me know. I’m happy to add to this post whenever something new comes up.